The new species Rhuda guayuyaco Henao, Bernal & Martínez, sp. nov. from the Andean-Amazonian piedmont of Colombia, is described and illustrated. The new species differs in the FW being pale pinkish brown, the dark area pale brown, with smooth margins, its distal margin perpendicular to the costa. It superficially resembles the Brazilian R. procas (Druce, 1894), from which it differs in the irregular band beyond the two subterminal lines near the tornus being a pale, thin irregular line, instead of a dark band. The male genitalia differ from those of other species in the size of the valve, the shape of the uncus and the shape and size of the aedeagus.