This article presents a longitudinal case study as part of a research that was carried out with a group of Colombian university professors of experimental sciences between the years 2019 to 2022. Due to the few offers of continuous training for university teaching development on contents of cognitive neuroscience, the purpose was to analyze the impact of the implementation of a training program in this line on the didactic knowledge of a group of teachers for teaching science. A statistical and descriptive analysis was performed using semi-structured interviews and a performance test before and after the intervention. It is concluded that the training program influenced the didactic knowledge about science teaching in the group of participating teachers, by generating adjustments in the way of linking their disciplinary knowledge with knowledge in didactics and cognitive Neuroscience in their teaching processes sciences at a higher level.