Objective: Acne vulgaris (AV) is a common skin disorder.Genotypic variations of antioxidantrelated genes may directly influence the function of AV-related genes by mitigating the risk of oxidative stress.This study aimed to investigate the impact of SOD1 +35A/C and GPx-3 +1494A/G gene polymorphisms in patients with AV. Materials and Methods:The study comprised 81 healthy controls and 81 AV patients.The GPx-3 +1494A/G genotype was evaluated using Allele-Specific Polymerase Chain Reaction (AS-PCR), while the SOD1 +35A/C genotype was analyzed through the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) technique.Results: Genotype and allele frequencies of the SOD1 +35A/C gene polymorphism differed significantly between the AV patients and the control group (χ 2 =13.9, df=2, p=0.001 and χ 2 =13.1, df=1, p=0.001, respectively).Individuals with the AA genotype, compared to those with the AC genotype, showed an increased incidence of AV (Odds Ratio [OR]=4.81,95% Confidence Interval [CI]=1.94-11.9,p=0.001).Individuals carrying the A allele were at a higher risk of AV compared to those with the C allele (OR=4.43,95% CI=1.87-10.4,p=0.001).The AC genotype and C allele were associated with a protective effect in the control group (OR=0.21,95% CI=0.08-0.52,p=0.001 and OR=0.23, 95% CI=0.10-0.54,p=0.001).However, no significant differences were observed in the GPx-3 +1494A/G genotype and allele frequencies between both groups. Conclusion:The findings of this study indicate a correlation between the SOD1 +35A/C polymorphism and an increased incidence of AV.