<i>Reinwardtia</i> Brauer &amp; Bergenstamm is a monotypic genus, with only <i>R. tachinina</i> described from Venezuela. Here, we revised the genus to include <i>Reinwardtia bicolor</i> sp. nov. from Colombia and Ecuador. To investigate the phylogenetic positioning of <i>Reinwardtia</i>, we present a morphological analysis, including 16 species of the Neotropical Reinwardtiini genera. We used 35 characters from male and female adults. Based on this phylogenetic analysis, <i>Reinwardtia</i> is a monophyletic genus, a sister-group of the monotypic <i>Synthesiomyia</i> Brauer &amp; Bergenstamm. http://zoobank.org:pub:F8495ED0-FBDA-4A91-9A76-40B708FC6997 http://zoobank.org/urn:lsid:zoobank.org:act:8BF649B3-C9F3-4B90-B120-1CE28F051F5A