We report two mitochondrial genomes of headstanders, derived from target capture and Illumina sequencing (HiSeq 2500 PE100). One trans-Andean species <i>Megaleporinus muyscorum</i> (mitochondrial consensus genome of 25 individuals) from Colombia and one cis-Andean species <i>M. obtusidens</i> from Argentina. Regarding <i>M. muyscorum</i>, mitochondrial genome has 13 protein-coding genes, 1 D-loop, 2 ribosomal RNAs, 21 transfer RNAs, and is 14,434 bp in length, for <i>M. obtusidens</i> mitochondrial genome has 13 protein-coding genes, 2 ribosomal RNAs, 22 transfer RNAs, and is 15,546 bp in length.