Here, we provide the two primary global connectivity datasets generated in the study titled "Functional connectivity of the world's protected areas", including the protected area isolation (PAI) metric for all included protected areas (i.e., effective resistance), provided as a csv file, and the map of global mammal movement probability (i.e., electrical current density), provided as a tif. We also include the nationally aggregated PAI values in National_PAI.csv. National PAI represents the median PAI value for each country, after excluding values equal to -1. Generation of these datasets relied on the following three external data sources: - Observed mammal movement data (0.95 quantile displacement distances over 10-days), predictor variables and the linear mixed effects model presented in: M. A. Tucker <em>et al.</em>, <em>Science</em>. <strong>359</strong>, 466–469 (2018). - The 2009 Global Human Footprint map presented in: O. Venter <em>et al.</em>, <em>Nat. Communications.</em> <strong>7</strong>, 1–11 (2016). - The May 2020 and April 2018 versions of the World Database on Protected Areas, found at: UNEP-WCMC and IUCN, Protected Planet: the World Database on Protected Areas (WDPA), Cambridge, UK, (available at www.protectedplanet.com). Please read the Readme.txt for file details and cite the following paper if you use these data: Brennan, A., R. Naidoo, L. Greenstreet, Z. Mehrabi, N. Ramankutty and C. Kremen. Functional connectivity of the world's protected areas. Science (2022).