Characterization techniques used for biopolymers analyses usually are spectroscopic and thermal, however, recently microscopic techniques have been gaining relevance in the characterization of this type of materials.This review provides an overview of the two most used spectroscopic techniques for the characterization of biopolymers: ultraviolet-visible spectroscopy (UV-Vis spectroscopy) and Fourier transform infrared spectroscopy (FTIR spectroscopy).Other techniques such as Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), spectroscopy magnetic resonance spectroscopy ( 1 H NMR and 13 C NMR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), differential thermal analysis (DTA), differential scanning calorimetry (DSC), and X-ray diffraction (XRD) are not discussed.This review does not intend to develop the techniques in depth regarding their physical principles and fundamentals.The objective of the document is to show the relevance of the techniques in a specific application: the characterization of biopolymers, specifically, starch.