Anaerobic digestion (AD) is a process of biochemical decomposition by anaerobic bacteria. Organic solid waste, subjected to anaerobic digestion, produces organic fertilizer and biogas, composed of methane, carbon dioxide, and other gases. The AM2 model describes the AD system and is the most widely used model for dynamic behavior analysis and control of AD processes. One of the most critical variables for system analysis is the concentration of volatile fatty acids (VFA). The behavior of the concentration of VFA depends on the dilution rate. This, in turn, depends on the performance parameters of producing and consuming VFA (k2 and k3). This paper presents a deterministic method to calculate the k2 and k3 parameters, from the dilution rate dynamics and Olson method for indirect validation of the parameters based on the direct validation of the concentration of VFA. Model results for VFA concentration showed an error of <0.4%. An error of <1.98% was found between the theoretical model and the experimental data. Overall, a global error of <2.37% gives a reliability to the proposed method to determine k2 and k3.