This chapter describes the basis and the basic criteria to design the detection path, given an illumination beam. The first part of the chapter describes the most basic detection schemes employed in different light sheet fluorescence microscopy (LSFM) systems and provides details about the components and their function in the imaging formation process. Then, the second part of the chapter describes the most advanced detection schemes, which are presented in the context of their respective applications. The chapter presents a review of the image formation using WFM will be presented, followed by the specific parameters needed to obtain an image in LSFM. It also presents the most advanced detection schemes are described, and some applications. Modern biological experiments require studying the interactions between the different elements involved in certain biological processes occurring in cells or tissue. Some experiments demand the acquisition of images with high contrast and/or resolution.