Cyperus rotundus (Cyperaceae) is a wild plant spread worldwide in tropical and subtropical areas, and it is known widely for its uses in conventional medicine to treat many ailments.In this research, the seeds of C. rotundus were subjected to extraction processes involving petroleum ether (a), dichloromethane (b), ethyl acetate (c), and methanol (d)using a Soxhlet apparatus.Additionally, the seeds were macerated with methanol (e),and an infusion (f) was prepared using water.These extracts were used to investigate the plant's antimicrobial, antioxidant, and enzyme-inhibitory activity.The antimicrobial activity of plant extracts was evaluated against ten pathogenic microorganisms, where inhibitory effect was observed against Candida tropicalis with 78.12 µg/mL MIC in (a).Total phenolic and total flavonoid contents were performed, and it is shown that (c)had high phenolic and flavonoid contents (62.32±1.21µg PEs/mg extract, 25.07±0.57µg QEs/mg extract, respectively).On the other hand, the antioxidant potential of the extracts was evaluated by DPPH and ABTS radical scavenging and CUPRAC activity methods.(d) was the most active (IC50=17.63±0.35µg/mL, and IC50=12.44±0.19µg/mL, A0.5=26.11±0.21µg/mL) regarding the DPPH, ABTS, and CUPRAC assays respectively.Acetylcholinesterase, butyrylcholinesterase, and tyrosinase enzyme inhibitoryactivities were conducted.(c) was the most active against acetylcholinesterase enzyme (IC50=49.29±1.44 µg/mL) compared with galantamine as a standard molecule (IC50=8.53±0.20).(d) showed high butyrylcholinesterase, and tyrosinase inhibitory activity (IC50=13.40±0.14, and 136.79±1.66respectively) compared with galantamine (IC50=38.66±0.49),and kojic acid (IC50=21.70±0.97)respectively.Phenolic compounds of the extracts were conducted by LC/HR-MS.While a high value of phenolics was detected as an ingredient of (c) compared to other extracts, kaempferol was detected from (c) as a major compound.Chlorogenic acid was detected as the major phenolic in (d), which showed the high antioxidant effect.C. rotundus which has high therapeutic potential, showed notable anticandidal, antioxidant, anti-butyrylcholinesterase, and anti-tyrosinase effects.