<title>Abstract</title> <bold>Background</bold><italic>:</italic> Cryptococcosis is a life-threatening disease caused by <italic>Cryptococcus neoformans</italic> or <italic>C. gattii</italic>. Autoantibodies (auto-Abs) neutralizing granulocyte-macrophage colony-stimulating factor (GM-CSF) in otherwise healthy adults with cryptococcal meningitis have been described since 2013. We searched for neutralizing auto-Abs in sera from Colombian patients with non-HIV related cryptococcosis in a retrospective national cohort collected from 1997 to 2016. <bold>Methods:</bold> We reviewed clinical and laboratory records and assessed the presence of neutralizing auto-Abs in 30 HIV (-) adults presenting cryptococcosis (13 by <italic>C. gattii</italic>, and 17 by <italic>C. neoformans</italic>). <bold>Results:</bold> We detected auto-Abs neutralizing GM-CSF in the plasma of 9 out of 13 (69%) patients infected with <italic>C. gattii</italic> and 1 out of 17 (6%) patients with <italic>C. neoformans</italic>. <bold>Conclusions:</bold> We report ten Colombian patients with cryptococcosis due to auto-Abs neutralizing GM-CSF. Nine of the ten patients were infected with <italic>C. gattii</italic>, and only one with <italic>C. neoformans.</italic>