Heavy metals have environmental importance due to their toxicity, for this reason, a wide variety of methods have been studied for their elimination in the water matrix. One of these methodologies is bioremediation, In this study, we investigated the technical feasibility of using the Trichonephila clavipes spider web as a biomaterial for iron removal in water by bioremediation. Experimental design were defined using the Response Surface Methodology (RSM), and the iron concentrations were measured before and after the experiment .We obtained an iron removal of 91.82%, using 28.09 h, 81.42 mg kg-1 of iron, and 0.062 g of spider web.