The antibacterial activity of the total ethanolic extract and fractions of Pelargonium graveolens against Candida albicans, Escherichia coli, Pseudomonas spp, Staphylococcus aureus and Bacillus spp. Methods: To measure the relative antimicrobial activity, the percentage of inhibition was calculated by the plate and well method. Results: It was established that the highest antimicrobial activity was presented by the oily extract without preference for Gram positive or Gram negative, the acetone fraction with a value of 58.53% inhibition. The hexane fraction has activity greater than 100% only against S. aureus. Conclusions: The extracts of P. graveolens present antimicrobial activity against Gram-positive and Gram-negative bacteria and yeasts, especially the oily extract, which presented the highest percentage of inhibition recorded against Escherichia coli, Staphylococcus aureus and Candida albicans, respectively; while for Pseudomonas aeruginosa and Bacillus subtilis, the extract that presented the highest percentage of inhibition was ethyl acetate.