The genus Rubus has the highest number of representatives within the Rosaceae family and is one of the most diverse in the Plantae kingdom, an economically important crop in the Andean region. In Colombia, the Castilla blackberry (Rubus glaucus Benth.) stands out among the cultivated species because of its various fruit size, color, and quality. This research aimed to conduct a callogenesis induction and then pro-embryogenic callus induction, where blackberry vitroplants were taken without stings in the multiplication stage; auxins such as 2,4-D and Picloram were used as growth regulators in six concentrations, with and without the presence of light. For callus multiplication, embryogenic calluses obtained during the induction phase were selected. These calluses were divided into portions approximately 0.5 cm in diameter with an initial approximate weight of 1.2 g. The analysis of variance yielded that statistically significant differences exist among the different treatments (p ˂ 0.05), where callus formation with the Picloram hormone at different concentrations, with the presence of light and darkness, indicated that the most callus formation was verified with the 1.0 mg/l concentration and under darkness, (4.92 calluses on average). For embryogenic callus multiplication, analysis of variance indicated that the highest weight averages corresponded to the 3.0 mg/l treatment of 2,4-D combined with 3.0 mg/l of BAP for a mean of 3.5 under light conditions and 2.79 under darkness. Keywords: callogenesis, hormones, embryogenesis, vitroplants. https://doi.org/10.55463/issn.1674-2974.50.10.1