The Amazon, with its multidimensional nature, diverse in organisms, cultures and in its biophysical aspects, plays a prominent role in regulating the climate and providing ecosystem services essential to life on Earth.Its complex nature leads to equally complex problems in the human-nature interface, bringing forth the urgency of promoting transdisciplinary discussions to create innovative, equitable, fair and sustainable solutions.Here, we present the opinion of professionals from academia, companies, funding agencies, non-governmental organizations, civil society and government, from different areas of knowledge (environmental, social, biological and health sciences) on how to perform an inclusive and transdisciplinary science with the objective of finding solutions for the sustainability and conservation of the Amazon.We applied a semi-structured questionnaire with five complementary discursive questions aiming to synthesize the multiple visions for the application of transdisciplinary science in the Amazonian context.Scientist training, appreciation of traditional knowledge, broader spaces and time for promoting knowledge integration and co-creation of policies involving multiple actors were identified both as the main bottlenecks and the main solutions for transdisciplinary science in the academic environment and in policy decision-making.Most interviewees had already gone through transdisciplinary experiences during their education, indicating that initiatives to promote and implement transdisciplinarity exist elsewhere.However, we emphasize that greater coordination is needed between large research groups and funding agencies so that these experiences can be linked to a long-term transformative process, essential for the consolidation of transdisciplinary practices across various sectors of society.