There is an increase in concern about the effects of environmental dangers on health related to toxic pollutants resulting from industrial activities, which are released into the aqueous environments in the whole world. Industrial dyes represent one of the major groups of toxic compounds used in different industries, including textile and leather. Releasing dyes into the water could cause vicious effects on the aqueous environment. This experiment aims to evaluate the efficiency of chemical treatment by Nano zero-valent iron (NZVI) of textile dye to investigate the histopathological effects in the liver, kidneys, and intestines of albino mice using histopathological diagnosis. Three groups of mice were exposed. The control group was given tap water, the second group was assigned untreated textile dye solution (acid red 315) at a concentration of (100)ppm, and the third group was given treated textile dye solution. Histopathological alterations were evaluated after 2 months from exposure. The results from treated dyes showed no histopathological changes. In contrast, untreated stains showed histopathological changes in all organs, represented by dilation of sinusoids and congestion of blood vessels and hydropic degeneration, necrosis and aggregation of inflammatory cells in the liver. In contrast, in the intestine, there is edema between mucosa and submucosa, degeneration and necrosis in the intestinal mucosa, atrophy in the muscular layer, and inflammatory cell aggregation in the submucosa and mucosa of the intestine. We conclude that the treatment of textile dye is sufficient and decreases its effect. Keywords: Histopathological alteration, Efficient evaluation, Textile dye acid red 315