In Colombia, studies have begun on the potentiality of fruits Sechium edule (Jacq.) Sw., as a plant matrix to design new food items. Therefore, it is necessary to develop tools allowing analysis of the structural changes resulting in the transformation processes of this matrix. We conducted a study on the morphoanatomical and histochemical characteristics of the S. edule, fruit smooth-light green varietal. This fruit morphology is proper varietal, but a wide weight variation was determined due to its size variation. The external fruit parts exhibit annular collenchyma in the subepidermal tissues. The middle part of the fruit is characterized by chlorenchyma, and reserve parenchyma with simple and semi-compound amyloplasts. Different types of calcium oxalate crystals are observed. Fruit tissues have abundant compounds such as inulin, pectins, starch and mucilage, that make this species a promising one for the production of functional foods.