In the last ten years, Colombia's growing number of paternity disputes has involved at least 65,400 fathers of different ethnic origins and ancestry. In this study, we determined how many erroneous paternity inclusions could be detected in Bogotá, the sixth most populous city in Latin America with a geographic area of 1,775 km2, by comparing the empirical results of 296 mothers (M) and son (S) duos and 219 alleged fathers (AF) (with 15 microsatellite markers). This comparison between each duo (M-S) and all unrelated males (AF) (i.e., all alleged fathers of the other cases) was performed with the implementation of a specially designed software module to perform test calculations of paternity and maternity, which resulted in 64,824 M/S/AF trios. For the 64,824 M/S/AF trios, fewer than three excluding STRs were found in 156 tests; that is, 0.2412% of the trio tests resulted in a non-exclusion of paternity. For 1 M-S duo (i.e., 0.3378%), one or more unrelated males with zero or three excluding STRs were detected. The same experiment was performed with unrelated AF-S duo cases; out of 65,262 duo tests generated, the proposed putative father could be confirmed in 375 tests; that is, 0.5746% of the duo tests resulted in a non-exclusion of the paternity. 55 Children were found with one additional presumed father, 29 with two additional AF, 11 with three additional AFs, two children with 4 and 5 additional AF, and one son with 6 and 7 additional P-P, respectively. Paternity probabilities ranged from 0.9974 to 99.999%, which shows that if the mother is excluded from the test, we can find matches in the genetic profiles of the alleged father and unrelated child. These results highlight the potential biases that can occur in motherless paternity cases using only STR analysis and recommend great caution in assigning verbal predicates such as "proven paternity" rather than continuing to use terms such as "non-excluded biological paternity".