Biodegradable biopolymers from species of the animal kingdom or their byproducts are sustainable for eco-friendly materials due to their plentiful supply and compatibility with the environment. This study focused on obtaining an active biodegradable material from chitosan, gelatin, and collagen from bocachico scales (Prochilodus magdalenae). The films were developed by compression molding and characterized according to moisture, water solubility, contact angle, mechanical properties, and structural properties. The results of the films showed a hydrophobic characteristic. Firstly, the chitosan-collagen (CS/CO) films showed a yellowish color, while those of gelatin-collagen (Gel/CO) were transparent and less soluble than those of gelatin-collagen (Gel/CO). Regarding mechanical properties, gelatin films showed greater rigidity and tensile strength than chitosan films. In addition, in the morphological analysis, it was observed that more homogeneous chitosan films were obtained by increasing the concentration of citric acid. In general, chitosan, gelatin and collagen extracted from bocachico (Prochilodus magdalenae) scales are an alternative in the application of films in the food industry.