Introduction: Bovine brucellosis is a zoonotic disease of considerable impact, especially in livestock countries, which can cause neurological and systemic complications in humans. Colombia is a country in which, despite its importance, there are few studies analyzing its behaviour and none developing maps with the use of geographic information systems to characterize it. Objective: to describe the temporal-spatial distribution of bovine brucellosis in Colombia 2009-2019. Methods: Retrospective cross-sectional descriptive study, based on weekly reports by the Colombian Agricultural Institute, of the surveillance of brucellosis in cattle on farms, in Colombia, from 2009 to 2019 (April). The data were converted into databases in Access 365®, and multiple epidemiological maps were generated with the Kosmo RC1®3.0 software coupled to shape files of all the departments. Results: in the study period, 510 properties were evaluated, with a median of 5 properties per department (minimum 0, maximum 129), 28 of the 33 departments or districts assessed (Bogotá, Amazonas, Guainía, Guaviare, San Andrés), were had a median of 53 farms evaluated per year. Of the total properties, 271 (53.14%), a range of positivity from 0 to 100%, with an average of 33.37% in those departments where it was evaluated, and an average of 49.39% per year, finding the highest accumulated positivity in Córdoba (100%), followed by Risaralda (77.8%), and Valle del Cauca (74.4%). Conclusions: GIS is an essential tool for understanding the temporospatial behaviour of zoonotic diseases in Colombia, as is the case of bovine brucellosis, with its potential implications for Human and One Health.