Introduction: follow-up of premature infants with oxygen in kangaroo mother programs includes evaluation, followup, and progressive withdrawal of supplemental oxygen.In these patients, the information on how to withdraw oxygen is limited.Objective: to describe the knowledge and practices of health personnel regarding oxygen management in kangaroo mother programs in Colombia.Material and methods: cross-sectional study.A questionnaire was applied, in virtual mode, to 55 health professionals who cared for patients in the Kangaroo Mother Programs of Colombia in the year 2020.Results: 60% of the participants did not have highrisk neonatal follow-up certification.90% claimed to know the deleterious effects of oxygen, and only 54.5% stated that there is an oxygen protocol in their institution.More than 80% perform a comprehensive approach to the patient, before withdrawing oxygen.Conclusion: health professionals from the kangaroo mother program show a good level of knowledge and practices in the management of ambulatory oxygen for premature infants, but it seems necessary to standardize its management.