Were determined mechanical milling parameters (impact velocity, collision energy, impact pressure, impacted powder temperature, etc.) to explain the structural transformation of titanium dioxide obtained by medium and high energy ball milling. The effects of milling time (6 hours and 24 hours) and the rotation velocity of the discs on the polymorphic transformation and the evolution of the powder characteristics of titanium dioxide during milling process were investigated using X-ray diffraction accompanied of Rietveld refinement. The results of our calculations and experiments confirm that polymorphic transformation of Anatase to Srilankite and Rutile takes place at high temperatures and high pressures in the milling. Furthermore, amorphization of crystalline phases occurred accompanied of decrease in the crystallite size (in the range of nanometers) and increase of microstrains as the milling time and energy increased.