The preservation of food in the global economy requires effective treatments with elements that do not leave residues in products of natural origin. This research used Trichoderma harzianum associated with Trichoderma koningii , Trichoderma lignorum , and Pichia kudriavzevii as biological controllers against the attack of fungal pathogens and ripening in Citrus sinensis Salustiana variety fruits. The design was descriptive experimental in random sets of Salustiana variety orange fruits to evaluate the efficacy of the products used during the postharvest phase, the development of the immersion technique, and the use of entomopathogenic fungi. Trichotropic (T. harzianum + T. koningii), Trichobiol (T. lignorum), P. kudriavzevii are efficient, functional, natural, and used for pest control. Four groups were evaluated, T4 corresponded to the control group. The other three contained the biological controller, the variables of pressure, temperature, and humidity were permanently monitored, with daily recording of the changes in each individual of the groups. The results show that the attack of the fungal pathogen Phytophthora citrophthora and the green mold caused by Penicillium digitatum occurs more quickly in the control group. The treatment with Trichoderma lignorum showed a better behavior. Keywords: Biological Controllers, Diseases, Entomopathogens, Fungal Pathogens DOI： https://doi.org/10.35741/issn.0258-2724.58.4.61