This study was conducted at one of the private orchards to determine the variety through the anatomical characteristics of the roots; three palm trees were selected for each variety, identical, 15-18 years of age. The anatomical characteristics of the roots of seven date palm varieties were studied. The studied traits were circumferential circle diameter, phloem bundle length, xylem bundle length, next xylem, epidermis, sub-epidermal, fibrous bundle, cortex, and the diameter of parenchymal cells. Results show that the studied varieties differed in the studied anatomical characteristics. The Khadrawi variety was significantly superior in the characteristic of the surrounding circle diameter compared to the rest of the studied varieties, The Barhi variety was significant in the cortex and phloem bundle length, The Asabee Al-Aroos variety was distinguished in the characteristic of epidermis thickness, and the lowest values were in the variety Halawi. The Halawi variety was distinguished by the thickness of the sub-epidermal area, and the lowest values were in the Barhi variety. Keywords: Anatomical study, roots, seven varieties, date palm (Phoenix dactylifera L.)