Abstract This study assessed the combined effect of partial feed replacement with the forage Gliricidia sepium (GS) as a source of protein and supplementation with the yeast Saccharomyces cerevisiae (SC) on blood chemistry parameters in broilers. Six treatments were used: T1 (control), T2 (control; no SC + 10% GS), T3 (control; no SC + 15% GS), and T4 (SC + 0% GS), T5 (SC + 10% GS), and T6 (SC + 15% GS). T5 exhibited an increase in CK (p = 0.0099) as compared to T1 (5567±733.2) IU/L. The use of SC and GS at 10% in broiler diet increased creatine kinase levels, which may be related to mild muscle or liver disturbances. Replacing part of the feed with GS increased protein availability, which correlated with increased availability of apoproteins for lipoprotein production.