Spinal anesthesia is generally used in pediatrics for postoperative analgesia; however, its use as an anesthetic technique in neonates, especially with high risk of complications (prematurity, pulmonary pathologies, among others) has been increasing exponentially in the last decade in order to avoid the risks generated by general anesthetics and airway manipulation.We present a case of a patient born at 30 weeks, with hyaline membrane disease, and subsequently, he must be taken for a rectal biopsy due to clinical suspicion of Hirschsprung's disease, applying spinal anesthesia as an anesthetic technique, without complications.