Abstract: The research focuses on the analysis of environmental variables and the operation of a greenhouse used for the production of fish and plants under aquaponic technology. It is important to mention the existence of studies on these techniques, although they do not represent and provide the totality of knowledge on the processes that occur in them, from their design and materiality. This work intends to make a contribution to sustainable production in aquaponic systems (greenhouse), studying the variables that most affect the development of plants and fish. The research was carried out in the aquaponic prototype of the "Los Robles" campus of the Fundación Universitaria de Popayán, Colombia. Initially, the effect of the physical and environmental parameters of the greenhouse was analyzed, such as design, production area, structure, climate and environment, which are variables that influence the productive performance of the greenhouses (fish and plants installed). With the results obtained from the analysis of environmental variables, improvements are proposed to optimize the production system. This proposal aims to contribute to food security and the social and productive reactivation of the communities in the region.