The experimental design herein was used to evaluate the influence of electrocoagulation parameters, such as initial pH and electrolyte support (Na2SO4) concentration, on Escherichia coli (E.coli) removal. The initial pH and Na2SO4 concentration influenced the response variable. E.coli removal is more efficient with a decrease in pH and an increase in Na2SO4 concentration. The predicted values from the empirical model were consistent with the experimental values. In a final experiment under optimal conditions (2.5 mg L-1 of Na2SO4 and initial pH 4.0), the electrocoagulation with aluminum electrodes was able to remove 1-log after 40 min and 1.9-log in a contact time of 90 min of E.coli. The study shows electrocoagulation as a promising alternative to remove microorganisms in water.