Glyphosate (GLY) is one of the most used herbicides worldwide. In rice paddy fields, it is usually applied for weed control during the pre-planting stage. Phosphate (P) fertilizers may enhance GLY displacement in the soil matrix. The objective of this study was to assess the effect of P on GLY transport and mineralization parameters in paddy soils from Espinal (CS1), Saldaña (CS2), and Villavicencio (CS3) in Colombia. GLY miscible displacement experiments were performed in disturbed soil columns, with and without the addition of P after the application of a pulse of N-(phosphonomethyl-14C) glycine (14C-GLY). Simultaneously, 14C-GLY mineralization was measured indirectly by quantifying the amount 14C-CO2 released daily. At the end of the experiment, the columns were divided into six horizontal sections and GLY-bound residues were determined in the soil. The addition of P decreased GLY retention time (in CS1 and CS2) and increased the total leached amount only in CS1 soil. More than 60% of the applied GLY was retained in the soil columns, indicating a high retention of the herbicide in the soil matrix. The addition of P did not affect the mineralization rate in any of the soils. The results show that, under the evaluated conditions, GLY was strongly retained in all the soils, and that the half-life adjust to bi-exponential model, implying that one fraction degrades rapidly due to being more bioavailable, and the other fraction present a slow rate of degradation.