BACKGROUND AND AIM: Organ�c-�norgan�c hybr�d nanofthey have h�gh stab�l�ty, low product�on cost, cheaper pur�f�cat�on processes, overcom�ng substrate/product �nh�b�t�on and easy recovery.We have synthes�zed tr�ple enzyme (amylase, l�pase, and pro-Cu(II)) and tested the�r ant�m�crob�al act�v�ty �n compar�son w�th ch�tosan.MATERIALS and METHODS: We synthes�zed ch�tosan from sh-r�mp shells and (TrpE@Cu(II)) us�ng l�pase, amylase, and protease enzymes.SEM analys�s, thermograv�metr�c analys�s (TGA), and the degree of deacetylat�on (%DD) were performed for ch�tosan character�zat�on, where SEM, FTIR, EDX, and XRD analys�s were performed for the character�zat�on of (TrpE@Cu(II)).Agar d�lut�on and broth d�lut�on methods were used to evaluate the ant�m�crob�al E. col� and S. aureus.zyme act�v�ty and stab�l�ty at h�gher pH and temperatures than each free enzyme.Follow�ng we evaluated ant�m�crob�al act�v�ty of (TrpE@Cu(II)) compared w�th ch�tosan, wh�ch �s natural, non-tox�c, b�odegradable, and b�ocompat�ble polymer w�th ant�ox�dant, ant�bacter�al, ant�fungal, ant�v�ral, and ant�-allerg�c propert�es because of �ts polycat�on�c structure.Ant�m�crob�al propert�es of ch�tosan have been spotl�ghted and enabled the �ndustry to use �t �n var�ous �ndustr�al products, �nclud�ng cosmet�cs, food, pharma-ceut�cals, and personal�zed med�c�ne.Our data showed that synt-hes�zed ch�tosan has the same character�st�c as a commerc�al one, where TrpE@Cu(II) showed s�gn�f�cant ant�m�crob�al act�v�ty on both gram-pos�t�ve and gram-negat�ve bacter�a, s�m�lar to ch�tosan f�rst t�me �n the l�terature.CONCLUSION: We showed TrpE@�hNFs, wh�ch can be cons�dered as a prom�s�ng approach for ant�b�ot�c res�stance and persona-l�zed med�c�ne.