Purpose: This study highlights the identification, resistance and susceptibility of microorganisms in healthcare workers’ hands. Methods: Several scientific databases were reviewed to summarize contributions of the past 10 years. A meta-analysis was conducted to assess bacteria on healthcare workers’ hands and their resistance and susceptibility profiles. Results: healthcare workers were colonized by 35 types of bacteria, highlighting S. aureus, Acinetobacter spp., and E. Coli. Although bacteria on healthcare workers’ hands was lower RR = 0.36-[0.17-0.77]; physicians acquired more bacteria RR = 0.87-[0.70-1.09]. Specifically, health personnel contracted S. aureus RR = 0.29-[0.27-0.31], Enterococcus spp. RR = 0.10-[0.07-0.13], S. epidermis RR= 0.55-[0.49-0.61], Acinetobacter spp. RR = 0.25-[0.22-0.27], E. Coli RR = 0.09-[0.07-0.11], Pseudomonas spp, RR = 0.07-[0.05-0.08] and Bacillus spp, RR = 0.08-[0.06-0.11]. Resistance and susceptibility profiles showed that S. aureus was susceptible to antibiotics; nevertheless, S. aureus was resistant to Ceftriaxone RR= 1.33-[1.01-1.74], Erythromycin RR= 2.05-[1.61-2.60] and Augmentin RR= 7.64-[5.46-10.68]. Conclusion: Detected microorganisms trigger pathologies of clinical importance such as skin infections, sepsis, gastroenteritis, among others; in addition, bacteria are the cause of pathologies of greater clinical importance, such as nosocomial pathologies due to work activity in the hospital environment, which require invasive treatment. The way of prescribing and using antibiotics needs to be changed, even if new drugs are developed, to reduce antibiotic resistance.