Chronosequences provide apt space-for-time substitutions for the study of ecological succession. A thorough understanding of ecological succession can guide restoration efforts in critical areas, such as highly fragmented tropical landscapes susceptible to dispersal and seedling establishment limitations. We evaluate changes in seed rain quantity and quality through ecological succession from seed traps set monthly from September 2017 to August 2018 in 18 plots (50 x 50 m) of a tropical humid forest chronosequence experiment in the lowland mountain physiographic landscape of Caquetá, Colombia. Three chronosequence groups were determined based on stand age (early-secondary forest, intermediate-secondary forest, and old-growth forest). Seed morphospecies richness and mean seed mass increased with stand age while abundance decreased, suggesting a change in species composition towards more, larger-seeded species characteristic of late-successional forests. However, species composition did not differ among stand age groups, likely due to the limitations of morphospecies identification. Peaks in seed rain (August-November) during mild rainy season months were driven by a small subset of small-seeded morphospecies, chiefly Eugenia sp. and Piptocoma discolor. Small seed rain peaks during dry season months suggest that abiotically-dispersed species contribute only a minority of collected seeds, with the exception of P. discolor. Structural variables of basal area (m2/ha), stem density, species density, and species diversity did not correlate with seed rain variables or differ among chronosequence groups due to unusually high variation in structure within groups. Overall, results suggest that early-secondary forests with at least 5 years of growth of native species are unlikely to be dispersal limited, indicating the potential for unassisted restoration in the landscape. However, it should be noted that this study did not include younger, more recently abandoned pastures which are at a higher risk of dispersal and seedling establishment limitations.