This protocol is designed for the design and adaptation of a LAMP primer set in order for it to be used with the QUASR technique. In order to do this, it requires an already designed (and hopefully experimentally validated) LAMP primer set. The steps indicated in this protocol are the ones we follow to adapt a previously validated primer set for their use in LAMP-QUASR reactions. QUASR is "a simple technique that allows closed-tube, target-specific detection, based on the inclusion of a dye-labeled primer that is incorporated into a target-specific amplicon if the target is present. A short, complementary quencher hybridizes to unincorporated primer upon cooling down at the end of the reaction, thereby quenching the fluorescence of any unincorporated primer" (Ball et al., 2016). Therefore, using QUASR-LAMP allows for specific sequence detection and reduces the false positives often associated with unspecific amplification, and it only requires a minor modification of one of the LAMP reaction primers. This protocol mostly focuses on the software and criteria we use to decide which primer of the set should be picked, modified with a fluorophore, and used as a template for quencher probe design. Some steps are based on the criteria used by Priye et al. (2017), with several modifications and additions of our own.