Knowledge about the diversity of coastal amphipods in the eastern tropical Pacific, including the Pacific coast of Colombia, is scarce. We characterized amphipod diversity in Bahía Málaga (intertidal and subtidal rocky shores, and sandy beaches) and Bahía Buenaventura (intertidal rocky shores, mangrove roots, and sandy beaches), two important estuaries in the central Pacific coast of Colombia. Sixteen species in eleven families were found; Hourstonius baciroa, Leptocheirus rhizophorae, Leucothoe alata, Elasmopus ocoroni, Melita bousfieldi, and Neomegamphopus heardi represent new records for Colombia.