Two studies addressed links between moral foundations, political attitudes, religiosity and perceptions of rape in Colombian respondents. In Study 1, the authors developed a Colombian version of the Moral Foundations Questionnaire (MFQ). In Study 2, the MFQ was further validated in relation to the acceptance of modern myths about sexual aggression (AMMSA), victim vs. perpetrator blaming regarding a rape vignette, political orientation and attitudes towards religion. Results showed that the Colombian MFQ was more reliable than an existing Spanish version. Endorsement of conservative foundations (ingroup loyalty, obedience to authority and purity) predicted right-wing political attitudes, higher religiosity, higher AMMSA and more rape victim blaming. Conversely, endorsement of liberal foundations (care and justice) predicted lower SDO and less rape victim blaming. These results replicate and extend previous work, including the critique that conservative foundations may reflect amoral attitudes rather than universal moral intuitions. Implications and requirements for further research are discussed.