Medical personnel run the risk of contracting infectious diseases caused by physical, microbiological and chemical agents because they are directly exposed to risk factors such as the presence of pathogens in the environment; For this reason, this article derives from research carried out in four hospital centers in the department of Santander (Colombia) that seeks to identify the most common fungal microorganisms, bacterial microorganisms that can transmit diseases through the enteral, respiratory, dermal and blood routes, the microorganisms that can cause lung infections, wound contamination (abscesses), folliculitis, cellulitis and otitis, among others, in operating room workers, as well as determining the most appropriate disinfection protocols and possible control strategies, determining the measures aimed at monitoring, containment and elimination of these sources of contamination.