This research tested the impact on the soil when implementing a Clean Intensive Livestock Production System (CILPS) via Voisin Rational Grazing (VRG). The methodology consists of the characterization of soil-grass-cattle management, including the soil chemical and biological analysis in the case study farm and reference land (comparison between extensive and CILPS-VRG systems). In addition, the VRG impact on agroecological management was evaluated by calculating the Soil Organic Matter Indicator (SOMI). Once the samples were processed, the T-Student test was applied, verifying the homogeneity of variances (F-Fischer) for the chemical variables and the Kruskal-Wallis test for the biological variables. The results identified substantial differences in some edaphic parameters such as organic matter, percentage of organic carbon, nitrogen, sulfur, and calcium. Moreover, to diversify nematodes and differences in the Rhabdhitids sp., the process characterized products from the natural organic fertilization in the soil with VRG. Finally, the calculation of the SOMI with a value of 41.6 for VRG indicates soil with no alteration or natural balance.