The study aimed to identify genomic regions of ROH hotspots/islands and genes of interest in Colombian Creole cattle. The Caqueteño Creole population used belongs to the University of Amazonia, Caquetá, Colombia. The dataset consisted of the genotypes of 127 animals genotyped with the Bovine HD 777,000 SNPs chip. The ROH islands were measured by the PLINK 1.9 program. Since genomic regions with the highest frequency of ROH occurrence are called hotspots. The present study identified ROH hotspots in BTA3, BTA5, BTA6, BTA8, BTA16, and BTA20 chromosomes. The identified genes associated with fertility, reproduction, muscular development, environmental resistance, and immunity traits suggest a natural selection effect on Caqueta creole breed over the years. Notwithstanding, more research needs to be carried out on the Caqueteño Creole breed to identify the specialized production traits of the population.