The aim of this work is to evaluate the survival of the strains Lactobacillus plantarum LPBM10 andLactobacillus casei ATCC 393 in substrates of cape gooseberry pulp and glucose isotonic solution(14% w/w). A concentration of 0.5 is inoculated (McFarland scale) and it is stored at 4°C during 15days. The counts were made from the dilution 108 in MRS agar, incubated at 37°C for 72 hours inmicroaerobic conditions. After 15 days counts in pulp and glucose solution are obtained, with averagesfor L. plantarum 3.23 ± 3.35 x 109 and 1.64 ± 1.57 x 109 CFU/mL and L. casei of 5.40 ± 2.36 x 108 and 7.34 ± 7.88 x 108 CFU/mL, respectively. The results show a good performance of both strains in thesubstrates, with levels above 106, approach considered in lacteous to define a food with probiotics. Thedevelopment of these foods or substrates, are alternatives for direct consumption or impregnationsolutions. This way allows the development of new products.