Microbial biotransformation is a relevant strategy to obtain high added value natural compounds under
 controlled environmentally friendly conditions. In this research work, the biotransformation of (R)-(+)-
 limonene using Penicillium digitatum DSM 62840 was evaluated. The study variables were the following:
 culture medium, pH, microorganism growth phase, substrate concentration, and inducing effect of the
 substrate. The results showed that a concentration of 14.7 mM limonene in the medium named malt
 yeast broth at a pH of 3.5, inoculated with induced spores at the early stage of the exponential growth,
 produces a high specificity and the highest concentration (1864 mg/L) of (R)-(+)-α-terpineol. The
 product obtained has a considerable potential industrial application.