Lactiplantibacillus plantarum is a Gram-positive bacterium that belongs to the lactic acid bacteria (LAB) group commonly used in the food industry. To use this microorganism, high biomass concentration is necessary, and models need to be established for predicting and improving its behavior along fermentation. However, current models for L. plantarum are applicable to only one substrate. The growth of a newly isolated strain L. plantarum 60-1 in a co-substrate (glucose and lactose) and lactic acid production in the batch process, were modeled in this work. Biomass growth was well described bydouble Monod kinetics. Substrate consumptions were modeled using two balance equations. Lactic acid was described with the Luedeking–Piret equation. No product inhibition was observed. Both glucose and lactose were metabolized in a concomitant way. This is the first report (as we know it) of a model includes dynamics of a dual limitation substrate glucoseand lactose in multiplicative effects on the growth of L. plantarum 60-1