In development economics of many countries, banana has been thought of as a key factor, however, banana trees suffer from susceptible to the main climatic conditions such as temperature, humidity, or solar radiation, resulting in a decrease in canopy and leaf area, which has an impact in bunch size and quality. In recent years, teledetection has emerged as a method to analyze the state of the plantation, where multispectral images captured by Unmanned Aerial Vehicle (UAV) are the main information source. This study set out to canopy extraction and estimation of a banana plantation from multispectral images taken at 35 meters height, which are transformed to HSV color field by red-edge band reflectance (REG) and near infrared (NIR). This allowed segmentation to separate the canopy from objects such as dry leaves, ground, and other elements. Finally, the results were compared with manual technique, resulting the proposed methodology has a significant accuracy and adjustment in the results..