Purpose: To Evaluate the inter-observer reproducibility of a mobile pediatric auto refractor in an infant Colombian population. Methodology: Quantitative longitudinal prospective study. Variables of age, sex, ocular history and refractive defect were measured. The sample size was estimated assuming as values of the intraclass correlation coefficient (ICC). The descriptive statistical analysis of the variables was performed and the ICC was calculated to evaluate the reproducibility of the data taken independently by two examiners. Results: 138 participants were included, median age was 42 months. 51% of the sample were females and hypermetropic astigmatism was the prevalent refractive defect with a 67.39% occurrence. The ICC for sphere, cylinder and axis, showed an almost perfect degree of agreement. Conclusions: A mobile pediatric auto refractor brings reliable data when evaluating the refractive status without cycloplegia in children aged 24-67 months. Although it is suggested to classify the refractive error as the average of three consecutive measurements and correlate the result with the clinical history of optometry to define the final formula. CE Notification: This article is available as a COPE accredited CE course. You may take this course for 1-hour credit. Read the article and take the qualifying test to earn your credit. Click here to Enroll (https://www.crojournal.com/reproducibility-of-mobile-auto-refractor-measurements-in-a-columbian-children-population) Please check COPE course expiry date prior to enrollment. The COPE course test must be taken before the course expiry date.