In this work, the distribution of Amanahyphes genus and A. saguassu Salles & Molineri species is expanded through the first records from Colombia and Perú. The departments where these specimens were found belong to the Amazon region of both countries, they are: Amazonas, Guaviare and Vaupés (Colombia); Madre de Dios (Perú). The species identified in this work was previously recorded only in Brazil and Venezuela.