Temperature as an abiotic factor is a parameter that considerably influences insect vector development due to its implications at a metabolic and an ethologic level, as well as vector competence. In the last decade, it had been suggested that endosymbionts play a role in the survival and replication of pathogens in these insects. Aedes albopictus is an insect vector known for the transmission of several arboviruses such as Chikungunya, Zika, Yellow Fever, and Dengue in tropical and subtropical regions. In the present study, the temperature preference of natural population of Ae. albopictus was evaluated as well as the presence of the endosymbiont Wolbachia. For the development of the study, 400 females of Ae. albopictus (F2) were collected from the municipality of Bucaramanga, department of Santander, Colombia and subjected to temperature preference tests in a temperature gradient device with stable temperatures from 21 °C to 34 °C, using 50 individuals for each replica. After 1 hour of exposure, the insects were labeled according to the temperature range they were recovered, and then stored at -20 ºC until the DNA extraction using the entire body of the insects to determine the presence of the Wolbachia bacteria by the amplification of the WSP gene. PCR products were sequenced to confirm the identity and phylogenetic location considering reference strains from other invertebrates. It was determined that, Ae. albopictus is distributed with different abundances from 21 ºC to 34 ºC, demonstrating that it is a species with great plasticity against the temperature variation. Specifically, it was possible to estimate a higher percentage of individuals (35.26%) between 21º and 23ºC, while 15% of individuals distributed from 23 to 29 °C. Results also showed that Wolbachia naturally infects Ae. albopictus from Santander. Analysis of the WSP gene sequences showed that the detected WSP gene haplotypes are located in supergroup B of the Wolbachia endosymbiont. A greater number of females infected (38.95%) with these bacteria were found in the temperature range of 21-23 ºC. This study provides evidence of natural Wolbachia infection in Ae. albopictus from Santander and a species temperature preference that can be involved in the presence of endosymbiotic bacteria such as Wolbachia.