This work characterized the bovine ruminal content obtained from a slaughterhouse plant at Cordoba, Colombia, aiming to establish energetic use and valorization potential. Samples of ruminal material (RM) were collected after benefit process, using two conditions as-fresh and oven-dried. Microbiological and bromatological quality, density, elemental and ultimate analysis, as calorific power values were measured, and complemented with optical microscopy, SEM, XEDS analysis, FTIR, TGA, and TGA-MS for both conditions. Combustion products from mixtures of natural gas and ruminal content were studied, using XRD and XRF techniques. Results showed that dried and sieved RM is a lignin-cellulosic heterogeneous biomass, with a real density of 164 kg/m3, a calorific power between 12 - 15 kJ/kg and rich in alkaline-earth elements. Fresh-state it has an important microbiological quality, because it does not have some pathogens of human risk. However, direct application as fertilizer had a negative effect on the growth of a model plant. RM might have a good potential for to be used in co-combustion, gasification and others energy processes. However, important considerations should be done about management of RM.