Objective: To detect and quantify the association between oropharyngeal cancer andperiodontal bacteria. Study Design: This is a patient-control study that was based on biopsies that were collected from tumor tissue obtained from patients with oropharyngeal squamous cell carcinoma between 2016 and 2017 and shed oral mucosal epithelial cells that were collected from controls using the Cepimax® brush, carrying out several brushings towards the posterior third edge of the tongue and the cheek. Porphyromonas gingivalis, Tannerella forsythia and Prevotella intermedia detection and absolute quantification was determined through q-PCR. Statistical analysis included a U- test, X2, Fisher’s exact test, odds ratio (OR) and Conditional logistic regression analysis and unconditional regression analysis (p<0.05). Results: A total of 48 donors older than 55 years old participated in this study. The population was distributed in 24 patients (cases) and 24 controls A robust association was established in cases and controls with significance regarding Prevotella intermedia (OR: 15.00) and Porphyromonas gingivalis (OR:11.00). However, multivariate analysis adjusted to the presence of different bacteria and the diverse confounding variables did not reveal significant differences for oropharyngeal cancer association. Conclusion: The bivariate analysis evidenced significant differences for Prevotella intermedia, suggesting it could be associated with oropharyngeal cancer.