Because of the frequent use of antibiotics, which leads to the occurrence of resistance by pathogenic bacteria, and for the benefit of oils and the ease of their availability with minor side effects, thus, the study aimed to investigate the anti-microbial activity of Sesame seed oil and Eucalyptus against P. aeruginosa bacteria.Ten clinical isolates of Pseudomonas aeruginosa were previously isolated from wounds and have multi-drug resistance to common antibiotics.The results of Sesame oil against the bacterial isolates showed no antibacterial activity not only in the agar well diffusion method but also in the disc diffusion method.At the same time, the findings showed that eucalyptus oil had an antibacterial activity with concentrations (20%,40%, 60%, 80%, and 100%) against these isolates.Further, the antibiotics susceptibility test results showed that bacterial isolates were resistant to Amikacin, sensitive to each of cefotaxime, chloramphenicol and levofloxacin, and the effect of Gentamicin differed against the tested bacteria.Our finding concludes that eucalyptus oil may be used as an alternative drug in the treatment as an external ointment for wound infection by Pseudomonas aeruginosa.