The psychometric properties of the Ages and Stages Questionnaire, Third Edition (ASQ-3) in a Colombian population were explored, including construct validity and convergent validity. The ASQ-3, in its Spanish version, and the Escala de Abreviada del Desarrollo (Abbreviated Development Scale - EAD-3, by its acronym in Spanish) were compared. Participants included 456 children selected at random from live births in the time period between 2014 and 2018. Results indicated that the ASQ-3 yielded valid results when compared with the EAD-3. Implications include using the ASQ-3 for accurate screening, especially when children are identified with fine motor deficits on the EAD-3.