This protocol describes the qualitative determination of IgG antibodies against the nucleocapsid (N) protein of SARS-CoV-2 in serum from domestic dogs and cats, the indirect ELISA (Enzyme-Linked Immunosorbent Assay) kit ID Screen® SARS-CoV-2 Double Antigen Multi-Species (IDvet, Grabels, France). The diagnostic kit detects antibodies against the nucleocapsid (N protein) of the SARS-CoV-2 virus. This test can be used on samples from dogs, cats, mink, ferrets, cattle, sheep, goats, horses and any other susceptible species requiring serum, plasma or whole blood samples.